Rat SRGN (Serglycin) ELISA Kit Packaging:96 wells Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GPNMB

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Substrate solution is then added and color develops in proportion to the amount of Rat VEGFA

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Rat SRGN (Serglycin) ELISA Kit Packaging:96 wells Standards or samples are addedThe Rat (SRGN) Serglycin ELISA Kit measures Serglycin in Rat samples. The plate has been pre coated with Rat SRGN antibody. SRGN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat SRGN Antibody is added and binds to SRGN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SRGN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate